FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24823507
Sequences flagged as poor quality0
Sequence length25-358
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT547200.22043621797677498No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC479390.1931193686693826No Hit
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG378620.1525247822557868No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT377580.15210582453156196No Hit
CAGGGAGGAAAAGTTTGGAAGAGGCAGAGAAATCCTGCTCTCCTCGCCTT375770.15137667695382445No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC335320.13508163854527083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA28955.894366E-630438.916300-309
GGGGGGG45004.747304E-629373.553310-319
GGGGGGT48253.2745927E-513697.512310-319
TTAAAAC102402.4582263E-512908.3320-329
CGGGTTT94956.340489E-59280.743300-309
TTTGGGG153353.9746374E-78619.5625310-319
GAAAATT179850.07349.513340-346
GGCCCCC181050.07300.8003340-346
GGGGGTT61850.07123.7397310-319
TAAACCC58756.54436E-86428.255290-299
TTTTCCC236801.314575E-45581.9673330-339
GCCCCCC123402.1417881E-45355.794340-346
GGAAATT192752.612828E-44571.7593300-309
TTTAAAC123350.04286.3716290-299
CAAAAAG308952.237682E-44278.394330-339
AAAATTT158600.04167.118340-346
GGCCCAA108754.158379E-44051.525320-329
CCCCCCT111304.3556682E-43958.7340-346
AACCCCC68654.9711333E-43850.8667320-329
TAAAACC182000.03631.346320-329
Produced by FastQC (version 0.11.5)