FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22838398
Sequences flagged as poor quality0
Sequence length25-356
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG424440.18584490908688078No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT395330.17309883118772168No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT384930.16854509672701212No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC366570.16050600396752873No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC360850.15800145001413848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT58000.041655.19295-299
TTCGACC98100.024627.941300-304
TCGACCA57359.23193E-621063.654300-304
AGAAACG71851.4490361E-516812.812290-294
TTTCGGA96300.012544.139290-294
TTCGGAA53250.011342.729290-294
GAAACGC61006.266455E-59901.645290-294
TTAACGA21450.09386.174275-279
CACTTCG93000.08659.502295-299
AACGCAC35750.08447.557290-294
AAACGCA47900.08406.406290-294
CGGAATT36751.0613826E-48217.691290-294
GCACTTC150406.3492334E-58031.919295-299
CTAGTTG25201.0333861E-77989.422275-279
TCGGAAT51350.07841.6133290-294
AGCGACG28004.515823E-87503.1094270-274
CGACCAG107950.07460.248300-304
ACTTCGA81800.07383.8667295-299
TAACGGT8001.47293E-47365.857265-269
CTTCGAC90550.05336.281295-299
Produced by FastQC (version 0.11.5)