FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814211
Sequences flagged as poor quality0
Sequence length25-367
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT289160.16231984677850733No Hit
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG286910.16105681020618876No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC269780.15144089176893663No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC263640.1479942053004761No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT262740.1474889906715487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG8500.036765.605285-289
GCTGGTT148300.012643.602300-304
GAAACCG70400.08878.059295-299
AGTAACG11001.3024453E-47748.124270-274
TAACGGT5701.4321877E-47476.2607260-264
TTTTTCA254850.07357.4497305-306
TTACCCG19151.7942562E-46527.5757275-279
ATACGTA7750.06048.5356280-284
TTTTCAA159751.18926284E-45868.6885305-306
GGTTTTT80201.7983379E-45844.9062300-304
TAACCGG21900.05707.9023275-279
ACTGAGT57952.3472449E-45392.7124285-289
CTGGTTT176751.4558443E-45304.232300-304
AGCATCG45750.04553.8457295-299
CGTCTCA38003.7006082E-44485.756280-284
GTTTTTT105303.1000708E-44451.6763290-294
CGTCGCT22254.3827618E-44213.5864280-284
CTACTTA41750.04082.8438280-284
GTTAGTT20950.04068.2273270-274
TTTCAGC160303.5921927E-43899.035295-299
Produced by FastQC (version 0.11.5)