FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20385776
Sequences flagged as poor quality0
Sequence length25-357
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG373700.18331409115846264No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT309780.1519588952610879No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC292990.1437227604188332No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT255590.12537663515973096No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC251090.12316921367133632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTAA60950.036093.098330
TTTAAAC87000.025285.914320-324
ACCCGGG93750.023465.326325-329
TTAAACC61500.017885.158320-324
CCCTTTA41450.017690.988315-319
TAAACCC48952.4335728E-514980.418320-324
GGCCCTT168800.013032.432315-319
CAAAGGG188250.011685.92310-314
CCGGGTT64800.011316.227325-329
CCCGGGT97653.228261E-511264.078325-329
GGGTTTA45006.855378E-59777.22325-329
AAAGGGC125405.3237694E-58771.429310-314
GCCCTTT127650.08616.821315-319
CGGGTTT72700.07564.9053325-329
ACGGTTG49000.07482.5664300-304
AACCCGG58950.07463.5264320-324
AAACCCG73850.07447.104320-324
CCTAGGA53451.4507171E-46859.6016300-304
CCTTTAA80650.06819.201315-319
CGGGTTA17752.0264338E-46196.829285-289
Produced by FastQC (version 0.11.5)