FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24234697
Sequences flagged as poor quality0
Sequence length25-368
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG592380.2444346632433655No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT530530.2189134033736836No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC490720.20248654233226024No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT382020.15763349547964225No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC353530.14587762331008308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACG40954.084679E-631666.568340-343
TCGTACG9207.4220025E-631322.365310-319
CGATGTA41600.020781.186330-339
TTTTCCG68451.1412927E-518944.426340-343
ACGTACG11900.015567.18280-289
CGTACGT14503.379978E-514905.127310-319
TTTCCGG71403.725288E-512107.805340-343
TTAACGG7908.527837E-59655.592270-279
GCTCGTA98750.08754.403310-319
GTTTAAA110058.849917E-57855.4956330-339
CGATGTT84000.07718.726300-309
AACCCGG61500.07028.4336290-299
TCGATCG25000.05763.3154320-329
TTCCGGG116401.9800737E-45570.215340-343
CTCGTAC78950.05474.9673310-319
TCCCGGG158350.05459.408330-339
CCAAAAT182952.445771E-44725.32320-329
ATGTTAC47150.04583.7607300-309
TAGTTAC17200.04434.836270-279
CGATCGA27953.995524E-44418.591320-329
Produced by FastQC (version 0.11.5)