FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21411643
Sequences flagged as poor quality0
Sequence length25-356
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG627080.29286869765202045No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT522750.24414287124066097No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC501580.23425572712939402No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT494730.23105653312078855No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC484290.22618068123030072No Hit
CCATCGTCGGCGCCCCAGACACCAGGGCGTCATGGTGGGCATGGGCCAGA455390.21268335176333736No Hit
TGCTGAAAAGGTCAAGGCCTTCTTGGCTGATCCATCTGCCTTTGTGGCTG220860.1031494874073886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGG114400.020017.48300-304
CGGGTTA17900.014214.771285-289
GTTAAAA56252.9655703E-513570.37290-294
GTCGAAT46300.012365.01295-299
TGTCGAA98450.011630.269295-299
AGTGGGC128700.08896.658300-304
AAGTTGA137255.88537E-58342.44295-299
GGGTTAA25600.07454.4263285-289
AAAATTT116951.2819054E-46527.0054290-294
TCCGGGT120200.06350.5264285-289
CGAATGA47900.05975.991295-299
CCGGGTT68600.05563.6533285-289
GGCCCAG450300.05085.498305-309
GTTGAGT75300.05068.614295-299
TTAAAAG78052.854609E-44890.0273290-294
GCGACAC27656.2357867E-74141.0483275-279
AGTTGAG143150.03999.301295-299
TCGAATG97200.03926.6113295-299
TTTCCCG72954.6548145E-43923.92280-284
TAGGGGG77300.03703.1042280-284
Produced by FastQC (version 0.11.5)