FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12262968
Sequences flagged as poor quality0
Sequence length25-363
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG207050.16884167030363284No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT194980.15899902862015133No Hit
TGGATGCTGGATAAATTGACCGGTGTGTTTGCTCCTCGTCCATCCACCGG193460.15775952444791505No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC177410.14467133894502537No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT150510.12273537694952805No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC145530.1186743698589118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTA17300.075323.61340-349
CGGTTAA11701.320801E-655687.965340-349
CCCTTTA44100.029548.717330-339
TAAACCG8309.970239E-626166.635340-349
TACCCGG20251.1869497E-521450.18320-329
TTAACGG4051.8990842E-520109.543300-309
GGGTTTA24601.751667E-517657.16310-319
GGTTAAA25701.9118213E-516901.406340-349
GTTAAAC24803.5604848E-513136.072340-349
GGTTAAC12904.49558E-512626.923300-309
GTTAACG9005.1579187E-512065.726300-309
CCTTTAA65954.1965763E-59879.442330-339
CGGGGGG38608.6253E-58439.757300-309
TAAACCC25909.708079E-58385.446330-339
CGGGTTA11651.374906E-47456.9297320-329
CTTTAAA62550.06944.3022330-339
GTTTAAC24401.6083133E-46675.709300-309
AAACCGG29701.7871652E-46267.9097340-349
TAACGGG11102.0326642E-46178.7505300-309
TTAAACC43451.8214453E-45998.1514340-349
Produced by FastQC (version 0.11.5)