FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27470116
Sequences flagged as poor quality0
Sequence length25-357
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT551960.20093107724772621No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC514210.1871888709898422No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT374380.13628628288282438No Hit
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG365300.1329808727418552No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC334490.12176504824369871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAA64300.045163.574300-304
AACGTCG16605.353435E-634988.168295-299
AGTAGGG53405.539925E-627191.178290-294
TCGGAAT70800.020508.602300-304
ACGTCGG28401.5681508E-817042.357295-299
GGAACGT87151.4755577E-516661.031295-299
CGGAATC75053.2827404E-512898.147300-304
CGTCGGA35850.011572.098295-299
GAATCCG59506.877609E-59761.404300-304
GTAGGGA74700.09718.936290-294
GAACGTC113200.06413.467295-299
TAGGGAA96750.05002.6147290-294
CAGAAAT314550.04616.1465295-299
TTTCCCA381452.82681E-43806.551290-294
TTTAATT132655.127129E-43648.7222285-289
CTAGGAC68350.03540.6216280-284
GGAATCC254350.02854.352300-304
TATACGA16100.00107993522732.9358260-264
AGGGAAC133309.664068E-42723.1975290-294
AGAAATC276550.02625.2195295-299
Produced by FastQC (version 0.11.5)