FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20884066
Sequences flagged as poor quality0
Sequence length25-358
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT328690.15738793393968398No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC320520.1534758604957483No Hit
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG277290.1327758684539687No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT222140.1063681756225057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCCC51208.636145E-621778.102330-339
ACCCCGG79402.0769257E-514043.31340-341
AAGGGGG98850.011280.109340-341
GGTTTTT104553.601041E-510665.125320-329
GGGTTTT162700.06853.3423330-339
GGGGCCC183801.1129381E-46066.5874310-319
CGTTATC10252.1803338E-46043.5713280-289
GGGTTAA24702.4116275E-45642.909290-299
GGTTTTA100451.9943668E-45550.2183330-339
GTTTTTA110052.3937719E-45066.0557320-329
GTTTTAA74250.05005.786330-339
CCCCCCA116502.682581E-44785.574330-339
CCCCGGG122252.95391E-44560.486340-341
GTAACGT14200.04362.4365280-289
GATATAC33504.4359837E-44160.593290-299
CCCGCGA33950.04105.445290-299
TTTGCAC98056.650141E-43249.184300-309
ACGTATA5107.7489146E-43239.0378280-289
GGGCCCT176156.132687E-43165.0264310-319
CCCCCAA121307.269924E-43064.1353330-339
Produced by FastQC (version 0.11.5)