FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28829563
Sequences flagged as poor quality0
Sequence length25-368
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG436300.15133770844878916No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT416120.14433794920859536No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC399700.13864240675448325No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC333500.11567986653144899No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT330070.11449011558031595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA141000.021230.906360-362
CGGGAAA101750.014710.357360-362
TTTAACC55405.6111014E-510807.068330-339
GTTTTAA103750.09617.856340-349
TTAACCC31700.09443.4330-339
TTTTAAC63901.119729E-47807.9233340-349
TAACCCG25950.07690.579330-339
CAAAAAT399550.07492.323350-359
AAAATTT144250.06917.5225350-359
GGGGGTT93300.06417.0586330-339
GGTTTAA95753.328945E-56252.862320-329
AGGGGGG169700.05880.0977340-349
TTTCCGG87156.5230306E-55724.9146310-319
TAACCAA65802.2162375E-45686.8496300-309
TAAAAAA213602.502348E-44671.5947340-349
GGGAAAA216357.616167E-54612.2144360-362
CGGATTC55203.6763205E-44519.26290-299
AAATTTC175950.04253.4214350-359
TTTCCCG101203.931661E-44225.8013350-359
AAACCCG129902.1578615E-43840.8494310-319
Produced by FastQC (version 0.11.5)