FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20767514
Sequences flagged as poor quality0
Sequence length25-360
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG298990.1439700486057214No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT240430.11577216223376567No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT235940.11361013167006893No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC232650.11202592664678111No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC224160.10793781094838795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG38650.028496.508300-304
CGGTTAA14450.08967.148290-294
TAAGGGC69600.07912.284300-304
AGTCCAG181200.06078.311300-304
GTCCGAG56050.05614.3237295-299
GGCTGGT406700.05416.228305-307
CCGCTAA18853.89874E-44494.5522285-289
CGTAAGG20704.7015006E-44092.8647285-289
AGGGAGT90905.858211E-43461.8574295-299
CTGGTCA215554.7061595E-43406.4487305-307
TCCGAGT53450.03170.1519295-299
TGTGTAA53957.664383E-43140.7712295-299
GTCCAGA182256.728467E-43021.646300-304
GCGTTCG20150.02803.0537280-284
GCTGGTC403200.02731.622305-307
AAGGGCT138509.7139453E-42650.7583300-304
CGCTAAG16250.02606.8403285-289
GTGTATA22203.45608E-112544.2136280-284
ACGAATA15600.00131113372477.2605275-279
GGGGTTA23100.00133477342445.0881280-284
Produced by FastQC (version 0.11.5)