FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22405923
Sequences flagged as poor quality0
Sequence length25-363
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG590080.2633589341532594No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT524200.2339559945823254No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC515720.23017128104921183No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT491240.2192455985856954No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC468270.20899384506498572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACC50004.290118E-511966.99320-329
TTAACCC29600.010107.255320-329
GGGGGGT61502.3433877E-67783.408340-346
CGGGGGG77654.7159174E-67705.7246340-346
CCCGGGG161354.2271742E-57416.7896340-346
CGTAACG7201.4677526E-47387.031280-289
TAACCCC29600.06738.17320-329
CCGGGGG120151.7462675E-56640.0273340-346
TTTTAAA126900.06286.8345330-339
GGGTTTA42701.8772073E-46227.9414330-339
GGGGGTT73550.05423.517340-346
TCGGGTA22402.7264087E-45342.4062290-299
CGGGGGA79450.03765.5725300-309
ACCCCCC59756.738102E-43338.0725330-339
GGTTTAA60506.9083064E-43296.6917330-339
CGGGTAT19958.001002E-43157.1006290-299
ACCCCAA201056.9358683E-42976.123310-319
TTAAAAC77407.366489E-72944.9958330-339
ACCCCGG87559.1378934E-72733.7498320-329
AACCCCC84759.670257E-72689.5889330-339
Produced by FastQC (version 0.11.5)