FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19562785
Sequences flagged as poor quality0
Sequence length25-355
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGGGTACTTCAGGGTCAGGATGCCACGCTTGCTCTGGGCCTCGTCG410800.20999055093638255No Hit
GGCAATGAGCGGTTCCGGTGTCCGGAGGCGCTGTTCCAGCCTTCCTTCCT343730.17570606639085384No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTGGTCTCGTGGATGCCGC326220.16675539806832207No Hit
GGCAATGAGCGGTTCCGCTGCCCTGAGGCACTCTTCCAGCCTTCCTTCCT290970.14873649125111787No Hit
GTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTGGATGCCAC280640.14345605699801947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAAAC83000.025433.182340-341
GGGTTTA44107.150551E-623933.719320-329
TTAAACC56301.1654112E-518747.371340-341
CGTAAGG31603.671331E-513360.469300-309
GGTTTAA55953.4528377E-512576.432320-329
CCCAAGG202200.010439.931330-339
GGGAAAT126155.8510916E-58366.841310-319
CGTTACG7050.07303.075280-289
CCCTTTA39650.06654.962330-339
TAAACCC47751.7603366E-46315.4946320-329
GTTTAAA84651.5806814E-46234.3594320-329
GGGGTTT403100.05236.8320-329
ACCCCAA202351.5054585E-45216.0957310-319
TAACCCC27350.04540.1743290-299
GGAAATT129103.6764672E-44087.8274310-319
CCAAGGC289250.03649.0132330-339
CCCCAAA146304.721306E-43607.2354310-319
AAATTTC126650.03333.524300-309
ATGGGCC136506.8497E-43092.9727300-309
CCTTTAA79750.02941.0715330-339
Produced by FastQC (version 0.11.5)