FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77915008
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAG654100.019.0940651
CAATGGA387350.018.599958
TGCGGGT337050.018.5394481
AGAGCGA307050.018.533671
GCGGGTG232500.018.51742
TGATTTC688300.018.4174751
AGCGCAA332950.018.37236410
AGACCGT319600.018.348323
AGGATTG613850.018.3447999
GTGCGCA377600.018.286463
ACAGGAT539700.018.2790587
TGCTACC413750.018.2282521
GCTGCAG417100.018.2259461
TAGACCG322450.018.1839122
CGGGAGA181400.018.183124
CTCGAGG542650.018.1588611
ACTAGAC292600.018.1443465
GACTTGA388150.018.1211439
GCGCGAT208800.018.1105751
ATAGACC455750.018.1089611
Produced by FastQC (version 0.11.5)