FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93052492
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA1622920.17440908514303946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA913000.019.2728821
GATGCTA787750.018.9848541
CGTGGGA586450.018.8353129
GAACACA621600.018.740125
CGAACGC465700.018.64930310
GCTGCAG733100.018.6413351
CCGAACG466250.018.6324989
AAAGGGA467200.018.588196
TGCTACA700300.018.5564543
AGGGAAA565750.018.524218
AGCAGGA649700.018.4866716
TCGAAAC842000.018.48022
ATGCTAC705950.018.4405562
AGAACAC624150.018.380094
GGAAACA570000.018.27890410
AGGATTG654400.018.2377829
AATCGTG567000.018.1677346
AGAACTC661550.018.1600658
CGAAACA855800.018.158113
CCCAAGG535950.018.1066364
Produced by FastQC (version 0.11.5)