FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005709154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93052492
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2180110.23428819079880206No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG981950.10552645919466616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4298350.081.200241
AGCAGTG4577000.076.057952
GTGGTAT5005450.069.057056
CAGTGGT5157100.067.0772254
TGGTATC5171350.066.722647
GGTATCA5209700.066.690078
AGTGGTA5253500.065.820015
GCAGTGG5344900.065.036653
GTATCAA5429700.064.172759
ATCAACG5094050.037.1665510-11
CAACGCA5146950.036.77182412-13
TACATGG4947300.036.20829822-23
GCAGAGT5199350.035.6379116-17
AGTACAT5248100.034.92494220-21
TCAACGC5048650.034.6575812-13
ACGCAGA5592700.033.93263614-15
CGCAGAG5159600.033.91129716-17
GTACATG5093100.033.13566222-23
CATGGGG2833600.033.07539424-25
GAGTACA5179100.033.01560220-21
Produced by FastQC (version 0.11.5)