FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201148588
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAT1824050.019.12479
TCCCGGA1830700.019.0579598
CGGACTG1343000.018.9333571
TCGAAGG1770950.018.8592572
CGAAGGT1775550.018.7970753
CCTCCCG1790750.018.7120866
ACGAGAG1154250.018.706459
CTCCCGG1796500.018.6766827
CACGAGA1156450.018.6725928
GCACGAG1099750.018.6716237
TGAGAGG1084300.018.6538541
GCCGGCA1186600.018.6497333
AAGAAAT1023650.018.6220322
TAGCCGG1495150.018.6169851
CATGGAA1565500.018.6122598
GACCTGG1322850.018.6090031
GCCGTCG1302550.018.5999939
CCGGATG1874250.018.59699210
CGCACGA860750.018.5837656
GCCGATG1102400.018.5416762
Produced by FastQC (version 0.11.5)