FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92977282
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA1633670.17570636233483358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA906750.019.2813111
GATGCTA786600.019.0037141
CGTGGGA587900.018.8418439
CCGAACG462800.018.7042169
CGAACGC463950.018.64923510
GCTGCAG719200.018.6388421
GAACACA627000.018.5891085
TGCTACA697150.018.5622253
AAAGGGA461200.018.4904796
AGGGAAA554900.018.4764238
AGCAGGA639050.018.4451186
TCGAAAC840450.018.4350952
ATGCTAC705100.018.4110772
AGAACAC626100.018.2876954
CGAAACA848200.018.244753
GGAAACA557650.018.24117510
AATCGTG565300.018.1878016
AGAACTC651750.018.1363228
AGGATTG644400.018.1332579
CCCAAGG532950.018.0941074
Produced by FastQC (version 0.11.5)