FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005709186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92210046
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTT797750.018.8223861
GAGGAGA306200.018.7457244
GTCGTGT649350.018.7406225
GGGTCGT615800.018.6357293
TGGGCGT390850.018.5969071
CGCTTCA687900.018.5130021
AGGAGAC339800.018.4728785
GGAGACC294150.018.3790956
GTGAGGA307700.018.3075052
CGTGTGG422050.018.2890017
AGCGTAT577050.018.1243271
AGATATG493500.018.1027268
TTCTAGA735200.018.0958357
CGTGAGC395950.018.0230481
TCGTGTG441750.017.9871886
CGGCCGA274100.017.9298829
TGACAAC467650.017.9274271
GGTCGTG440450.017.8920334
ACGGGTC728800.017.8896181
CTGCGGA269950.017.8613281
Produced by FastQC (version 0.11.5)