FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005709421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005709421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850195
Sequences flagged as poor quality0
Sequence length10-85
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGGAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC42440.22938122738414057Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC29760.16084791062563675Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTAGGAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT29440.15911836319955464TruSeq Adapter, Index 16 (100% over 37bp)
GAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCAT18970.10252973335243042TruSeq Adapter, Index 16 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)