FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263905
Sequences flagged as poor quality0
Sequence length10-85
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6450.2444061309941077Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5120.19400920785888862Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA4840.18339932930410563No Hit
GGCCGGTAATACGACTCACTATA4670.17695761732441598No Hit
GGCCGGTAATAC4020.15232754210795552No Hit
GCCGGTAATACGACTCACTATA4010.15194861787385613No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3950.14967507246925976Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATA3880.147022602830564No Hit
GCCGGTAATACGACTCACTATAGGGGCACGAC3340.12656069418919688Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC3150.11936113374130843Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATAC3140.11898220950720903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)