FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258631
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTACCGGCC5130.1983520923632511No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4540.1755396684852164Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC4380.1693532484504951No Hit
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3110.12024853942489494Clontech Universal Primer Mix Short (95% over 21bp)
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC3050.11792863191187446Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2860.11058225812064292Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)