FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265152
Sequences flagged as poor quality0
Sequence length10-85
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6800.25645667390779625Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5310.200262490948588Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA4560.1719768283852281No Hit
GGCCGGTAATACGACTCACTATA4500.1697139753801593No Hit
GCCGGTAATA4080.15387400434467777No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3920.14783972966449432Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC3670.1384111754767077No Hit
GCCGGTAATACGACTCACTATA3560.1342626116340816No Hit
GCCGGTAATAC3520.13275404296403573No Hit
GCCGGTAATACGACTCACTATAGGGGCACGAC3260.12294834660873763Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC2710.10220552739560705Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)