FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265152
Sequences flagged as poor quality0
Sequence length10-61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTACCGGCC5380.2029024861211682No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4490.16933683321264784Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC4330.1633025585324644No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC3230.12181692010620324Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3130.1180454984310886Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3000.11314265025343954Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)