FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264324
Sequences flagged as poor quality0
Sequence length10-85
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6760.25574673506756856Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5480.207321317776668Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA4600.1740288433891739No Hit
GGCCGGTAATA4440.16797566622781132No Hit
GCCGGTAATA4100.15511266475991586No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3850.14565457544528684Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATA3650.13808810399358362No Hit
GGCCGGTAATAC3440.13014330896929527No Hit
GCCGGTAATAC3250.1229551610901772No Hit
GCCGGTAATACGACTCACTATAGGGGCACGAC3040.11501036606588882Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)