FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558810
Sequences flagged as poor quality0
Sequence length10-85
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC13300.23800576224477016Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC9560.17107782609473704Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA8900.1592670138329665No Hit
GGCCGGTAATACGACTCACTATA8440.15103523558991427No Hit
GCCGGTAATACGACTCACTATA7530.1347506308047458No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC7510.13439272740287395Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATA7080.1266978042626295No Hit
GGCCGGTAATAC6840.1224029634401675No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC5960.10665521375780677Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)