FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558810
Sequences flagged as poor quality0
Sequence length10-61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13410.23997423095506523No Hit
TATTACCGGCC10550.18879404448739284No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC7590.1358243410103613Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC7370.13188740358977113No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC5620.10057085592598558Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)