FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545950
Sequences flagged as poor quality0
Sequence length10-85
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC13160.2410477149922154Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC9930.18188478798424765Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA8400.1538602436120524No Hit
GGCCGGTAATACGACTCACTATA8050.14744940012821686No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC7130.13059804011356352Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATA6870.12583569923985713No Hit
GCCGGTAATACGACTCACTATA6340.11612785053576336No Hit
GGCCGGTAATAC6270.11484568183899625No Hit
GCCGGTAATACGACTCACTATAGGGGCACGAC5610.10275666269804927Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)