FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545950
Sequences flagged as poor quality0
Sequence length10-61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.20056781756571115No Hit
TATTACCGGCC9910.18151845407088563No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC7850.14378606099459657Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC6720.1230881948896419No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC6440.1179595201025735Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5820.10660316878835059Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)