FastQCFastQC Report
Thu 23 Jun 2022
EGAF00005711349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568976
Sequences flagged as poor quality0
Sequence length10-85
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC13340.2344562863811479Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC10560.1855965805236073Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA8650.15202750203875032No Hit
GGCCGGTAATACGACTCACTATA8290.14570034588453643No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC7220.12689463175951182Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATA6710.11793116054104216No Hit
GCCGGTAATACGACTCACTATA6550.11511909113916931No Hit
GGCCGGTAATAC6330.11125249571159415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)