FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568976
Sequences flagged as poor quality0
Sequence length10-61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.228129130226934No Hit
TATTACCGGCC10390.18260875678411745No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC8220.14447006552121708Clontech Universal Primer Mix Short (95% over 21bp)
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC6640.11670088017772279Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC6360.11177975872444532No Hit
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5930.10422232220691206Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5710.1003557267793369Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)