FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104109
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC3140.30160696961838074Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA1890.18154050082125464No Hit
GGCCGGTAATA1800.17289571506786158No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC1720.16521146106484552Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT1690.16232986581371447Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC1400.13447444505278122Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT1400.13447444505278122Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC1240.11910593704674909Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC1220.11718487354599506Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)