FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102497
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2640.2575685142004156Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC2580.2517146843322244No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1900.18537127915939003Clontech Universal Primer Mix Short (95% over 21bp)
GAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1350.13171117203430344Clontech Universal Primer Mix Short (95% over 21bp)
AGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1190.11610095905246007Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1190.11610095905246007Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC1140.111222767495634No Hit
TATAGTGAGTCGTATTACCGGCC1030.1004907460706167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)