FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104320
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC3210.3077070552147239Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA2010.19267638036809814No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT1900.18213190184049077Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGAC1820.17446319018404907Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA1660.15912576687116564No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT1480.1418711656441718Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC1330.12749233128834356Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC1260.12078220858895705Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCT1150.11023773006134968Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATA1110.10640337423312883No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC1060.10161042944785276Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)