FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104320
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2690.25786042944785276Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC2480.23773006134969327No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2090.2003450920245399Clontech Universal Primer Mix Short (95% over 21bp)
AGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1520.14570552147239263Clontech Universal Primer Mix Short (95% over 21bp)
GAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1280.1226993865030675Clontech Universal Primer Mix Short (95% over 21bp)
AGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1210.11598926380368098Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGC1190.11407208588957055No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1160.11119631901840492Clontech Universal Primer Mix Short (95% over 21bp)
CGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1060.10161042944785276Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)