FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103642
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC3020.29138766137280253Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGAC1800.1736747650566373Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA1700.1640261669979352No Hit
GGCCGGTAATACGACTCACTATA1630.15727214835684375No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT1380.1331506532100886Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC1250.12060747573377588Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT1180.11385345709268443Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC1180.11385345709268443Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC1170.11288859728681422Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)