FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225053
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6150.27326896331086453Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA3990.17729157131875603No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3690.16396137798651872Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA3470.15418590287621137No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC3320.14752080621009273Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT2880.12796985598947805Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2520.11197362399079328Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT2490.11064060465756954Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCT2290.10175380910274467Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)