FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218235
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6120.2804316447865833Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA3680.16862556418539648No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3540.16221046120008248Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA3300.15121314179668707No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC3080.14113226567690793Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT2890.1324260544825532Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT2480.11363896716841937Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2380.10905675075033794Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC2270.10401631269044836Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)