FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218235
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5140.23552592388938529Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC4760.21811350150067588No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3110.14250693060233233Clontech Universal Primer Mix Short (95% over 21bp)
GAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2400.10997319403395422Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2320.10630742089948908Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)