FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228741
Sequences flagged as poor quality0
Sequence length10-85
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6360.27804372631054336Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC3740.1635037006920491Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGAC3670.1604434709999519Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA3580.15650888996725554No Hit
GGCCGGTAATACGACTCACTATA3430.1499512549127616No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT3350.1464538495503648Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT2500.10929391757489912Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCT2430.10623368788280196Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)