FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224609
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC6030.26846653517891095Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT3490.1553811289841458Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA3480.15493591084952074No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC3350.14914807509939496Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA3260.1451411118877694No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC3190.142024584945394Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2450.1090784429831396Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)