FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224609
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4910.21860210410090422Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC4540.20212903311977704No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3740.16651158234977226Clontech Universal Primer Mix Short (95% over 21bp)
GAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2520.112194969925515Clontech Universal Primer Mix Short (95% over 21bp)
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2340.10418104350226394Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)