FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185823
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5330.28683209290561446Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC3900.20987714115044964No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3710.19965235735081235Clontech Universal Primer Mix Short (95% over 21bp)
AGTGAGTCGTATTACCGGCC3080.16574912685727816No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2630.1415325336476109Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC2180.11731594043794365No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC2180.11731594043794365Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2160.11623964740640287Clontech Universal Primer Mix Short (95% over 21bp)
CGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2060.11085818224869903Clontech Universal Primer Mix Short (95% over 21bp)
AGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2050.11032003573292866Clontech Universal Primer Mix Short (95% over 21bp)
CGTGCCCCTATAGTGAGTCGTATTACCGGCC1940.10440042405945443Clontech Universal Primer Mix Short (95% over 21bp)
TCGTATTACCGGCC1890.10170969148060251No Hit
TATTACCGGC1880.10117154496483212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)