FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182697
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5990.32786526325008075Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA5720.3130866954575061No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC4000.21894174507517913Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA2930.16037482826756871No Hit
GCCGGTAATACGACTCACTATA2890.15818541081681692No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC2840.15544863900337716Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2640.1445015517496182Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT2400.13136504704510749Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT2380.1302703383197316Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACT2250.12315473160478825No Hit
GGCCGGTAATAC1970.10782880944952572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)