FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182697
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC5040.2758665987947257Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC3730.20416317728260452No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3280.1795322309616469Clontech Universal Primer Mix Short (95% over 21bp)
AGTGAGTCGTATTACCGGCC2920.15982747390488075No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2820.1543539302780013Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC2490.136291236309299No Hit
AGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2390.13081769268241952Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2140.11713383361522084Clontech Universal Primer Mix Short (95% over 21bp)
CGTGCCCCTATAGTGAGTCGTATTACCGGCC2040.11166028998834136Clontech Universal Primer Mix Short (95% over 21bp)
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC1970.10782880944952572Clontech Universal Primer Mix Short (95% over 21bp)
CTATAGTGAGTCGTATTACCGGCC1850.10126055709727033No Hit
TCGTATTACCGGCC1830.10016584837189446No Hit
CGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1830.10016584837189446Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)