FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185683
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATA5960.3209771492274468No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5660.3048205813133136Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGAC4040.21757511457699413Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC3150.16964396309839888Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA3030.16318133593274559No Hit
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2830.1524102906566568Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATA2520.13571517047871912No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT2310.12440557293882586Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC2140.11525018445415036No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT2080.1120188708713237Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACT1980.10663334823327929No Hit
GCCGGTAATACGACTCACTATAGGGGCACGAC1950.10501769144186598Clontech Universal Primer Mix Short (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)