FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185683
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4840.2606592956813494Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC3960.21326669646655858No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3430.18472342648492324Clontech Universal Primer Mix Short (95% over 21bp)
AGTGAGTCGTATTACCGGCC2950.15887291782231006No Hit
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2830.1524102906566568Clontech Universal Primer Mix Short (95% over 21bp)
AGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2470.1330224091596969Clontech Universal Primer Mix Short (95% over 21bp)
CGTGCCCCTATAGTGAGTCGTATTACCGGCC2390.12871399104926137Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC2330.12548267746643474No Hit
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGC2110.11363452766273703Clontech Universal Primer Mix Short (95% over 21bp)
TCGTATTACCGGCC2020.10878755728849707No Hit
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2020.10878755728849707Clontech Universal Primer Mix Short (95% over 21bp)
GAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1860.10017072106762599Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)