FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184581
Sequences flagged as poor quality0
Sequence length10-85
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5600.30338983969097577Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA5590.30284807212009907No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC4270.23133475276436904Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA2960.16036320097951579No Hit
GGCCGGTAATACGACTCACTATAGGGGCAC2780.1506113847037344Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATA2670.1446519414240902No Hit
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC2450.13273305486480189Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATAC2270.12298123858902055No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT2240.1213559358763903Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT2210.11973063316376008Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGAC2000.10835351417534848Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACT1900.10293583846658107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)