FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184581
Sequences flagged as poor quality0
Sequence length10-61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC4790.2595066664499596Clontech Universal Primer Mix Short (95% over 21bp)
TATTACCGGCC3950.21399819049631327No Hit
GTCGTGCCCCTATAGTGAGTCGTATTACCGGCC3430.18582627681072267Clontech Universal Primer Mix Short (95% over 21bp)
AAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2860.15494552527074834Clontech Universal Primer Mix Short (95% over 21bp)
AGTGAGTCGTATTACCGGCC2780.1506113847037344No Hit
AGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC2460.13327482243567865Clontech Universal Primer Mix Short (95% over 21bp)
TATAGTGAGTCGTATTACCGGCC2160.11702179530937637No Hit
TCGTATTACCGGCC2000.10835351417534848No Hit
CGTGCCCCTATAGTGAGTCGTATTACCGGCC1990.10781174660447175Clontech Universal Primer Mix Short (95% over 21bp)
CGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1930.1045611411792113Clontech Universal Primer Mix Short (95% over 21bp)
CGGAAGAGCGTCGTGCCCCTATAGTGAGTCGTATTACCGGCC1870.10131053575395084Clontech Universal Primer Mix Short (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)