FastQCFastQC Report
Sat 18 Jun 2022
EGAF00005711443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005711443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389168
Sequences flagged as poor quality0
Sequence length10-85
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC12060.3098918718908029Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATA10380.26672285491098957No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGAC7910.20325412161328782Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCAC5700.14646630761008098Clontech Universal Primer Mix Short (95% over 21bp)
GCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTCC5130.1318196768490729Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATA5040.12950705093944004No Hit
GCCGGTAATACGACTCACTATA4970.1277083418986145No Hit
GGCCGGTAATACGACTCACTATAGGGGCACGACGCT4540.11665912921925749Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTT4240.10895037618714797Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACTATAGGGGCACGACGCTCTTC4040.10381120749907494Clontech Universal Primer Mix Short (95% over 21bp)
GGCCGGTAATACGACTCACT3990.1025264153270567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)